Detection of 6K1 as a mature protein of 6 kDa in plum pox virus-infected Nicotiana benthamiana.
Identifieur interne : 000329 ( Main/Exploration ); précédent : 000328; suivant : 000330Detection of 6K1 as a mature protein of 6 kDa in plum pox virus-infected Nicotiana benthamiana.
Auteurs : Angelika Waltermann [Allemagne] ; Edgar Maiss [Allemagne]Source :
- The Journal of general virology [ 0022-1317 ] ; 2006.
Descripteurs français
- KwdFr :
- Escherichia coli (MeSH), Feuilles de plante (virologie), Polyprotéines (analyse), Polyprotéines (isolement et purification), Protéines virales (analyse), Protéines virales (génétique), Protéines virales (isolement et purification), Tabac (virologie), Virus de la variole du prunier (composition chimique), Virus de la variole du prunier (génétique).
- MESH :
- analyse : Polyprotéines, Protéines virales.
- composition chimique : Virus de la variole du prunier.
- génétique : Protéines virales, Virus de la variole du prunier.
- isolement et purification : Polyprotéines, Protéines virales.
- virologie : Feuilles de plante, Tabac.
- Escherichia coli.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Polyproteins, Viral Proteins.
- chemistry : Plum Pox Virus.
- genetics : Plum Pox Virus, Viral Proteins.
- chemical , isolation & purification : Polyproteins, Viral Proteins.
- virology : Plant Leaves, Tobacco.
- Escherichia coli.
Abstract
The RNA genome of Plum pox virus (PPV) encodes one large polyprotein that is subsequently cleaved into mature viral proteins. One of the products of proteolytic processing, the 6K1 protein, has not yet been identified in vivo for any member of the genus Potyvirus. In this study, 6K1-specific polyclonal antiserum was raised against PPV 6K1 expressed in Escherichia coli as a translational fusion with the N terminus of avian troponin C and an unusual metal-binding cluster of troponin T-1. For detection of 6K1 in vivo, a pPPV-H6K1-NAT infectious clone was constructed, enabling concentration of histidine-tagged 6K1 by affinity chromatography. Affinity-purified 6K1 was detected in locally infected Nicotiana benthamiana leaves at 4, 7 and 14 days post-inoculation (d.p.i.) and, in addition, in systemically infected leaves at 14 d.p.i., 6K1 was detected exclusively as a protein of 6 kDa and no polyprotein precursors were identified with the raised anti-6K1 antiserum.
DOI: 10.1099/vir.0.81873-0
PubMed: 16847134
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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wicri:level="3"><nlm:affiliation>Institute of Plant Diseases and Plant Protection, University of Hannover, Herrenhäuser Straße 2, 30419 Hannover, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Plant Diseases and Plant Protection, University of Hannover, Herrenhäuser Straße 2, 30419 Hannover</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16847134</idno><idno type="pmid">16847134</idno><idno type="doi">10.1099/vir.0.81873-0</idno><idno type="wicri:Area/Main/Corpus">000317</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000317</idno><idno type="wicri:Area/Main/Curation">000317</idno><idno type="wicri:explorRef" wicri:stream="Main" 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uniqKey="Maiss E" first="Edgar" last="Maiss">Edgar Maiss</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Plant Diseases and Plant Protection, University of Hannover, Herrenhäuser Straße 2, 30419 Hannover, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Plant Diseases and Plant Protection, University of Hannover, Herrenhäuser Straße 2, 30419 Hannover</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Escherichia coli (MeSH)</term><term>Plant Leaves (virology)</term><term>Plum Pox Virus (chemistry)</term><term>Plum Pox Virus 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xml:lang="fr"><term>Polyprotéines</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plum Pox Virus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Virus de la variole du prunier</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plum Pox Virus</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term><term>Virus de la variole du prunier</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Polyproteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Polyprotéines</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Feuilles de plante</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Plant Leaves</term><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Escherichia coli</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The RNA genome of Plum pox virus (PPV) encodes one large polyprotein that is subsequently cleaved into mature viral proteins. One of the products of proteolytic processing, the 6K1 protein, has not yet been identified in vivo for any member of the genus Potyvirus. In this study, 6K1-specific polyclonal antiserum was raised against PPV 6K1 expressed in Escherichia coli as a translational fusion with the N terminus of avian troponin C and an unusual metal-binding cluster of troponin T-1. For detection of 6K1 in vivo, a pPPV-H6K1-NAT infectious clone was constructed, enabling concentration of histidine-tagged 6K1 by affinity chromatography. Affinity-purified 6K1 was detected in locally infected Nicotiana benthamiana leaves at 4, 7 and 14 days post-inoculation (d.p.i.) and, in addition, in systemically infected leaves at 14 d.p.i., 6K1 was detected exclusively as a protein of 6 kDa and no polyprotein precursors were identified with the raised anti-6K1 antiserum.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16847134</PMID><DateCompleted><Year>2006</Year><Month>09</Month><Day>15</Day></DateCompleted><DateRevised><Year>2020</Year><Month>10</Month><Day>06</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-1317</ISSN><JournalIssue CitedMedium="Print"><Volume>87</Volume><Issue>Pt 8</Issue><PubDate><Year>2006</Year><Month>Aug</Month></PubDate></JournalIssue><Title>The Journal of general virology</Title><ISOAbbreviation>J Gen Virol</ISOAbbreviation></Journal><ArticleTitle>Detection of 6K1 as a mature protein of 6 kDa in plum pox virus-infected Nicotiana benthamiana.</ArticleTitle><Pagination><MedlinePgn>2381-2386</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1099/vir.0.81873-0</ELocationID><Abstract><AbstractText>The RNA genome of Plum pox virus (PPV) encodes one large polyprotein that is subsequently cleaved into mature viral proteins. One of the products of proteolytic processing, the 6K1 protein, has not yet been identified in vivo for any member of the genus Potyvirus. In this study, 6K1-specific polyclonal antiserum was raised against PPV 6K1 expressed in Escherichia coli as a translational fusion with the N terminus of avian troponin C and an unusual metal-binding cluster of troponin T-1. For detection of 6K1 in vivo, a pPPV-H6K1-NAT infectious clone was constructed, enabling concentration of histidine-tagged 6K1 by affinity chromatography. Affinity-purified 6K1 was detected in locally infected Nicotiana benthamiana leaves at 4, 7 and 14 days post-inoculation (d.p.i.) and, in addition, in systemically infected leaves at 14 d.p.i., 6K1 was detected exclusively as a protein of 6 kDa and no polyprotein precursors were identified with the raised anti-6K1 antiserum.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Waltermann</LastName><ForeName>Angelika</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institute of Plant Diseases and Plant Protection, University of Hannover, Herrenhäuser Straße 2, 30419 Hannover, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maiss</LastName><ForeName>Edgar</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Institute of Plant Diseases and Plant Protection, University of Hannover, Herrenhäuser Straße 2, 30419 Hannover, Germany.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Gen Virol</MedlineTA><NlmUniqueID>0077340</NlmUniqueID><ISSNLinking>0022-1317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020815">Polyproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017801" MajorTopicYN="N">Plum Pox Virus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020815" MajorTopicYN="N">Polyproteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>7</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>9</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>7</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16847134</ArticleId><ArticleId IdType="doi">10.1099/vir.0.81873-0</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Basse-Saxe</li></region><settlement><li>Hanovre</li></settlement></list><tree><country name="Allemagne"><region name="Basse-Saxe"><name sortKey="Waltermann, Angelika" sort="Waltermann, Angelika" uniqKey="Waltermann A" first="Angelika" last="Waltermann">Angelika Waltermann</name></region><name sortKey="Maiss, Edgar" sort="Maiss, Edgar" uniqKey="Maiss E" first="Edgar" last="Maiss">Edgar Maiss</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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